Improvement of photostability using feedback from single-molecule fluorescence time trace
Laboratoire de Photonique Quantique et Moléculaire, ENS Cachan, UMR 8537 CNRS, 61 avenue du Président Wilson, 94235 Cachan Cedex, France
2 Laboratoire EM2C, UPR 288 CNRS, École Centrale Paris, Grande Voie des Vignes, 92295 Châtenay-Malabry Cedex, France
Corresponding author: email@example.com
Single-molecule fluorescence experiments are limited by photobleaching. Some photobleaching pathways involve photoexcitation from the triplet state. We report an optical feedback technique that yields a strong enhancement in the photostability of single fluorescent dye molecules. This is achieved by detecting in real time quantum jumps to the triplet state and then quickly switching off laser illumination while the molecule remains in its triplet state.
© EDP Sciences, 2008