Effect of different agents onto multidrug resistant cells revealed by fluorescence correlation spectroscopy

C. Boutin; Y. Roche; R. Jaffiol; J.-M. Millot; C. Millot; J. Plain; R. Deturche; P. Jeannesson; M. Manfait; P. Royer

doi:10.1051/anphys:2008027

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Volume 32 / No 2-3 (2007)

Ann. Phys. Fr., 32 2-3 (2007) 139-141

Abstract

Issue		Ann. Phys. Fr. Volume 32, Number 2-3, 2007 COLOQ'1010th conference on lasers and quantum optics


Page(s)		139 - 141
Section		Short contributions
DOI		https://doi.org/10.1051/anphys:2008027
Published online		23 May 2008

Ann. Phys. Fr., Vol. 32, N°2-3 2007, pp. 139-141

Effect of different agents onto multidrug resistant cells revealed by fluorescence correlation spectroscopy

C. Boutin¹, Y. Roche², R. Jaffiol¹, J.-M. Millot², C. Millot², J. Plain¹, R. Deturche¹, P. Jeannesson², M. Manfait² and P. Royer¹

¹ LNIO (Laboratoire de Nanotechnologie et d'Instrumentation Optique), Institut Charles Delaunay, CNRS FRE 2848, Université de Technologie de Troyes, 12 rue Marie Curie, B.P. 2060, 10010 Troyes Cedex, France
² Unité MéDIAN (Médicament, Dynamique Intracellulaire, Architecture Nucléaire), CNRS UMR 6142, Faculté de Pharmacie, Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51096 Reims Cedex, France

Corresponding author: rodolphe.jaffiol@utt.fr

Abstract

Fluorescence correlation spectroscopy (FCS), which is a sensitive and non invasive technique, has been used to characterize the plasma membrane fluidity and heterogeneity of multidrug resistant living cells. At the single cell level, the effects of different membrane agents present in the extra-cellular medium have been analyzed. Firstly, we reveal a modification of plasma membrane microviscosity according to the addition of a fluidity modulator, benzyl alcohol. In the other hand, revertant such as verapamil and cyclosporin-A appears to act more specifically on the slow diffusion sites as microdomains.